Anti-MLLT3 Polyclonal Antibody (HA633014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HA633014

品牌

abinScience

描述

Anti-MLLT3 Polyclonal Antibody (HA633014) is a rabbit polyclonal antibody detecting Protein AF-9; ALL1-fused gene from chromosome 9 protein; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; YEATS domain-containing protein 3; MLLT3; AF9; YEATS3 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human MLLT3 (Met1-Thr148).

靶标

Protein AF-9; ALL1-fused gene from chromosome 9 protein; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; YEATS domain-containing protein 3; MLLT3; AF9; YEATS3

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P42568, A2AM29

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Protein AF-9 (MLLT3) is a ~63 kDa protein. Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr). Also recognizes and binds histone H3 acetylated and butyrylated at 'Lys-9' (H3K9ac and H3K9bu, respectively), but with lower affinity than crotonylated histone H3.

1. Lin, C. et al. (2010) Molecular cell 37, 429-37. PMID: 20159561

2. He, N. et al. (2010) Molecular cell 38, 428-38. PMID: 20471948

3. Li, Y. et al. (2014) Cell 159, 558-71. PMID: 25417107

4. Li, Y. et al. (2016) Molecular cell 62, 181-193. PMID: 27105114

5. Zhang, Q. et al. (2016) Structure (London, England : 1993) 24, 1606-12. PMID: 27545619

6. Li, X. et al. (2018) Nature chemical biology 14, 1140-1149. PMID: 30374167

7. Klein, BJ. et al. (2018) Nature communications 9, 4574. PMID: 30385749

9. Becht, DC. et al. (2025) Nature structural & molecular biology 32, 709-718. PMID: 39794553

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-AF9 polyclonal antibody at 1ug/mL on various samples.
Lane 1: THP-1 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Hela cell lysate

参考文献

产品说明书

Anti-MLLT3 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MLLT3 Polyclonal Antibody (HA633014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MLLT3 Polyclonal Antibody (HA633014)

概述

图片

参考文献

产品说明书

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