Anti-ARFIP1 Polyclonal Antibody (HA647014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HA647014

品牌

abinScience

描述

Anti-ARFIP1 Polyclonal Antibody (HA647014) is a rabbit polyclonal antibody detecting Arfaptin-1; ADP-ribosylation factor-interacting protein 1; ARFIP1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human ARFIP1 (Ile117-Gln373).

靶标

Arfaptin-1; ADP-ribosylation factor-interacting protein 1; ARFIP1

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P53367, G5E8V9, Q9JHU5

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Arfaptin-1 (ARFIP1) is a ~41 kDa protein. Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network. Mechanistically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold. Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs. In turn, ARFs induce fission. Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells.

1. Gehart, H. et al. (2012) Developmental cell 23, 756-68. PMID: 22981988

2. Kanoh, H. et al. (1997) The Journal of biological chemistry 272, 5421-9. PMID: 9038142

Note

For research use only

图片

参考文献

产品说明书

Anti-ARFIP1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ARFIP1 Polyclonal Antibody (HA647014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ARFIP1 Polyclonal Antibody (HA647014)

概述

图片

参考文献

产品说明书

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