Anti-DAP Polyclonal Antibody (HA684014)

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概述
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参考文献
产品说明书

概述

货号

HA684014

品牌

abinScience

描述

Anti-DAP Polyclonal Antibody (HA684014) is a rabbit polyclonal antibody detecting Death-associated protein 1; DAP-1; DAP; DAP1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human DAP (Ser2-Lys102).

靶标

Death-associated protein 1; DAP-1; DAP; DAP1

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P51397, Q91XC8, Q5EAE6, Q9QX67

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Death-associated protein 1 (DAP) is a ~11 kDa protein. Ribosome-binding protein involved in ribosome hibernation, a process during which ribosomes are stabilized in an inactive state and preserved from proteasomal degradation. Acts via its association with eiF5a (EIF5A and EIF5A2) at the polypeptide exit tunnel of the ribosome, preventing mRNA translation. Involved in ribosome hibernation in the mature oocyte by preventing mRNA translation, leading to ribosome inactivation. Ribosomes, which are produced in large quantities during oogenesis, are stored and translationally repressed in the oocyte and early embryo. Also acts as a negative regulator of autophagy.

1. Koren, I. et al. (2010) Current biology : CB 20, 1093-8. PMID: 20537536

2. Deiss, LP. et al. (1995) Genes & development 9, 15-30. PMID: 7828849

Note

For research use only

图片

参考文献

产品说明书

Anti-DAP Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-DAP Polyclonal Antibody (HA684014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-DAP Polyclonal Antibody (HA684014)

概述

图片

参考文献

产品说明书

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