Anti-RPA1 Polyclonal Antibody (HA686014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HA686014

品牌

abinScience

描述

Anti-RPA1 Polyclonal Antibody (HA686014) is a rabbit polyclonal antibody detecting N-terminally processed; RPA1; REPA1; RPA70 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human RPA1 (Ile187-Met616).

靶标

Replication protein A 70 kDa DNA-binding subunit; RP-A p70; Replication factor A protein 1; RF-A protein 1; Single-stranded DNA-binding protein; Replication protein A 70 kDa DNA-binding subunit, N-terminally processed; RPA1; REPA1; RPA70

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P27694, Q8VEE4

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Replication protein A 70 kDa DNA-binding subunit (RPA1) is a ~68 kDa protein. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response.

1. Gupta, R. et al. (2007) Blood 110, 2390-8. PMID: 17596542

2. Haahr, P. et al. (2016) Nature cell biology 18, 1196-1207. PMID: 27723717

3. Bass, TE. et al. (2016) Nature cell biology 18, 1185-1195. PMID: 27723720

4. Lin, YL. et al. (1998) The Journal of biological chemistry 273, 1453-61. PMID: 9430682

5. Maréchal, A. et al. (2014) Molecular cell 53, 235-246. PMID: 24332808

6. Sleeth, KM. et al. (2007) Journal of molecular biology 373, 38-47. PMID: 17765923

7. Aboussekhra, A. et al. (1995) Cell 80, 859-68. PMID: 7697716

8. DeMott, MS. et al. (1998) The Journal of biological chemistry 273, 27492-8. PMID: 9765279

9. Grudic, A. et al. (2007) Nucleic acids research 35, 7267-78. PMID: 17959650

10. Sharma, R. et al. (2022) Blood 139, 1039-1051. PMID: 34767620

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-RPA1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Lncap cell lysate

参考文献

产品说明书

Anti-RPA1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-RPA1 Polyclonal Antibody (HA686014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-RPA1 Polyclonal Antibody (HA686014)

概述

图片

参考文献

产品说明书

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