Anti-BRCC3 Polyclonal Antibody (HA695014)

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100ug

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概述
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产品说明书

概述

货号

HA695014

品牌

abinScience

描述

Anti-BRCC3 Polyclonal Antibody (HA695014) is a rabbit polyclonal antibody detecting Lys-63-specific deubiquitinase BRCC36; 3.4.19.-; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36; BRCC3; BRCC36; C6.1A; CXorf53 in ELISA, IHC, WB. Suitable for Human and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human BRCC3 (Val12-Thr223).

靶标

Lys-63-specific deubiquitinase BRCC36; 3.4.19.-; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36; BRCC3; BRCC36; C6.1A; CXorf53

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P46736, A5PJP6

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Lys-63-specific deubiquitinase BRCC36 is a ~36 kDa protein. Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates.

1. Cooper, EM. et al. (2009) The EMBO journal 28, 621-31. PMID: 19214193

2. Feng, L. et al. (2010) The Journal of biological chemistry 285, 30982-8. PMID: 20656690

3. Zheng, H. et al. (2013) Cell reports 5, 180-93. PMID: 24075985

4. Zeqiraj, E. et al. (2015) Molecular cell 59, 970-83. PMID: 26344097

5. Dong, Y. et al. (2003) Molecular cell 12, 1087-99. PMID: 14636569

6. Chen, X. et al. (2006) Cancer research 66, 5039-46. PMID: 16707425

7. Sobhian, B. et al. (2007) Science (New York, N.Y.) 316, 1198-202. PMID: 17525341

9. Shao, G. et al. (2009) Genes & development 23, 740-54. PMID: 19261746

10. Feng, L. et al. (2009) Genes & development 23, 719-28. PMID: 19261748

Note

For research use only

图片

参考文献

产品说明书

Anti-BRCC3 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-BRCC3 Polyclonal Antibody (HA695014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-BRCC3 Polyclonal Antibody (HA695014)

概述

图片

参考文献

产品说明书

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