Anti-GPR4 Polyclonal Antibody (HA696014)

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概述

货号

HA696014

品牌

abinScience

描述

Anti-GPR4 Polyclonal Antibody (HA696014) is a rabbit polyclonal antibody detecting G-protein coupled receptor 4; G-protein coupled receptor 6C.l; GPR6C.l; GPR4 in ELISA, IHC, WB. Suitable for Human, Bovine, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human GPR4 (Ile284-Gln362).

靶标

G-protein coupled receptor 4; G-protein coupled receptor 6C.l; GPR6C.l; GPR4

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P46093, Q1JQB3, P50132

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

G-protein coupled receptor 4 (GPR4) is a ~40 kDa protein. Proton-sensing G-protein coupled receptor activated by extracellular pH, which is required to monitor pH changes and generate adaptive reactions. Activated by an optimal pH of 6.8-7.2. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. GPR4 is mainly coupled to G(s) G proteins and mediates activation of adenylate cyclase activity. May also couple with G(i), G(q) and G(12)/G(13) G proteins.

1. Ludwig, MG. et al. (2003) Nature 425, 93-8. PMID: 12955148

2. Tobo, M. et al. (2007) Cellular signalling 19, 1745-53. PMID: 17462861

3. Rowe, JB. et al. (2021) The Journal of biological chemistry 296, 100167. PMID: 33478938

4. Howard, MK. et al. (2025) Cell 188, 671-687.e20. PMID: 39753132

5. Ma, Y. et al. (2025) Nature communications 16, 605. PMID: 39799123

6. Yue, X. et al. (2025) Cell research 35, 423-436. PMID: 40211064

7. Guo, L. et al. (2025) Molecular cell 85, 1658-1673.e7. PMID: 40215959

8. Chen, LN. et al. (2025) Molecular cell 85, 1640-1657.e8. PMID: 40215960

Note

For research use only

图片

参考文献

产品说明书

Anti-GPR4 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-GPR4 Polyclonal Antibody (HA696014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-GPR4 Polyclonal Antibody (HA696014)

概述

图片

参考文献

产品说明书

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