Anti-POLR2G Polyclonal Antibody (HA732014)

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50ug
100ug

数量：

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概述
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概述

货号

HA732014

品牌

abinScience

描述

Anti-POLR2G Polyclonal Antibody (HA732014) is a rabbit polyclonal antibody detecting DNA-directed RNA polymerase II subunit RPB7; RNA polymerase II subunit B7; DNA-directed RNA polymerase II subunit G; RNA polymerase II 19 kDa subunit; RPB19; POLR2G; RPB7 in ELISA, IHC, WB. Suitable for Rat, Human, Mouse, Bovine, and Zebrafish.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human POLR2G (Met1-Ser172).

靶标

DNA-directed RNA polymerase II subunit RPB7; RNA polymerase II subunit B7; DNA-directed RNA polymerase II subunit G; RNA polymerase II 19 kDa subunit; RPB19; POLR2G; RPB7

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P62489, P62487, P62488, Q5E9B8, Q7ZW41

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

DNA-directed RNA polymerase II subunit RPB7 (POLR2G) is a ~19 kDa protein. Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. POLR2G/RPB7 is part of a subcomplex with POLR2D/RPB4 that binds to a pocket formed by POLR2A/RPB1, POLR2B/RPB2 and POLR2F/RPABC2 at the base of the clamp element. The POLR2D/RPB4-POLR2G/RPB7 subcomplex seems to lock the clamp via POLR2G/RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft.

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-POLR2G polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate
Lane 4: A375 cell lysate
Lane 5: MCF-7 cell lysate
Lane 6: U251 cell lysate
Lane 7: SW620 cell lysate
Lane 8: A549 cell lysate

参考文献

产品说明书

Anti-POLR2G Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-POLR2G Polyclonal Antibody (HA732014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-POLR2G Polyclonal Antibody (HA732014)

概述

图片

参考文献

产品说明书

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