Anti-AFG2A Polyclonal Antibody (HA744014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HA744014

品牌

abinScience

描述

Anti-AFG2A Polyclonal Antibody (HA744014) is a rabbit polyclonal antibody detecting ATPase family gene 2 protein homolog A; 3.6.4.10; AFG2 AAA ATPase homolog A; Ribosome biogenesis protein SPATA5; Spermatogenesis-associated factor protein; Spermatogenesis-associated protein 5; AFG2A; SPAF; SPATA5 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human AFG2A (Thr351-Leu893).

靶标

ATPase family gene 2 protein homolog A; 3.6.4.10; AFG2 AAA ATPase homolog A; Ribosome biogenesis protein SPATA5; Spermatogenesis-associated factor protein; Spermatogenesis-associated protein 5; AFG2A; SPAF; SPATA5

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q8NB90, Q3UMC0

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

ATPase family gene 2 protein homolog A (AFG2A) is a ~97 kDa protein. ATP-dependent chaperone part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability. Required for replication fork progression, sister chromatid cohesion, and chromosome stability. The ATPase activity is specifically enhanced by replication fork DNA and is coupled to cysteine protease-dependent cleavage of replisome substrates in response to replication fork damage. Uses ATPase activity to process replisome substrates in S-phase, facilitating their proteolytic turnover from chromatin to ensure DNA replication and mitotic fidelity. Plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles.

1. Krishnamoorthy, V. et al. (2024) Cell 187, 2250-2268.e31. PMID: 38554706

2. Ni, C. et al. (2022) Cell reports 38, 110597. PMID: 35354024

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-AFG2A polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: LO2 cell lysate
Lane 3: Mouse liver lysate
Lane 4: Mouse brain lysate

参考文献

产品说明书

Anti-AFG2A Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-AFG2A Polyclonal Antibody (HA744014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-AFG2A Polyclonal Antibody (HA744014)

概述

图片

参考文献

产品说明书

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