Anti-RNF220 Polyclonal Antibody (HA773014)

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概述
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参考文献
产品说明书

概述

货号

HA773014

品牌

abinScience

描述

Anti-RNF220 Polyclonal Antibody (HA773014) is a rabbit polyclonal antibody detecting E3 ubiquitin-protein ligase RNF220; 2.3.2.27; RING finger protein 220; RING-type E3 ubiquitin transferase RNF220; RNF220; C1orf164 in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human RNF220 (Lys482-Leu566).

靶标

E3 ubiquitin-protein ligase RNF220; 2.3.2.27; RING finger protein 220; RING-type E3 ubiquitin transferase RNF220; RNF220; C1orf164

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q5VTB9, Q08DV5, Q6PDX6

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

E3 ubiquitin-protein ligase RNF220 is a ~62 kDa protein. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of SIN3B. Independently of its E3 ligase activity, acts as a CTNNB1 stabilizer through USP7-mediated deubiquitination of CTNNB1 promoting Wnt signaling. Plays a critical role in the regulation of nuclear lamina.

1. Ma, P. et al. (2014) Molecular and cellular biology 34, 4355-66. PMID: 25266658

2. Sferra, A. et al. (2021) Brain : a journal of neurology 144, 3020-3035. PMID: 33964137

Note

For research use only

图片

参考文献

产品说明书

Anti-RNF220 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-RNF220 Polyclonal Antibody (HA773014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-RNF220 Polyclonal Antibody (HA773014)

概述

图片

参考文献

产品说明书

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