Anti-NCOA2 Polyclonal Antibody (HA816014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HA816014

品牌

abinScience

描述

Anti-NCOA2 Polyclonal Antibody (HA816014) is a rabbit polyclonal antibody detecting Nuclear receptor coactivator 2; NCoA-2; Class E basic helix-loop-helix protein 75; bHLHe75; Transcriptional intermediary factor 2; hTIF2; NCOA2; BHLHE75; SRC2; TIF2 in ELISA, IHC, WB. Suitable for Human, Rat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human NCOA2 (Val109-Cys380).

靶标

Nuclear receptor coactivator 2; NCoA-2; Class E basic helix-loop-helix protein 75; bHLHe75; Transcriptional intermediary factor 2; hTIF2; NCOA2; BHLHE75; SRC2; TIF2

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q15596, Q9WUI9, Q61026

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Nuclear receptor coactivator 2 (NCOA2) is a ~159 kDa protein. Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as a RORA coactivator to specifically modulate G6PC1 expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3.

1. Druker, J. et al. (2013) Molecular and cellular biology 33, 2116-27. PMID: 23508108

2. Voegel, JJ. et al. (1996) The EMBO journal 15, 3667-75. PMID: 8670870

3. Voegel, JJ. et al. (1998) The EMBO journal 17, 507-19. PMID: 9430642

4. Musille, PM. et al. (2012) Nature structural & molecular biology 19, 532-S2. PMID: 22504882

5. Musille, PM. et al. (2016) The Journal of biological chemistry 291, 1411-26. PMID: 26553876

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-NCOA2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Jurkat cell lysate

参考文献

产品说明书

Anti-NCOA2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-NCOA2 Polyclonal Antibody (HA816014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-NCOA2 Polyclonal Antibody (HA816014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价