Anti-FOXK1 Polyclonal Antibody (HB309014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HB309014

品牌

abinScience

描述

Anti-FOXK1 Polyclonal Antibody (HB309014) is a rabbit polyclonal antibody detecting Forkhead box protein K1; Myocyte nuclear factor; MNF; FOXK1; MNF in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human FOXK1 (Ala86-Arg395).

靶标

Forkhead box protein K1; Myocyte nuclear factor; MNF; FOXK1; MNF

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P85037, P42128

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Forkhead box protein K1 (FOXK1) is a ~75 kDa protein. Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis, muscle cell differentiation and autophagy. Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context. Together with FOXK2, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen. Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4. Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate.

1. Freddie, CT. et al. (2007) Nucleic acids research 35, 5203-12. PMID: 17670796

2. Wang, W. et al. (2015) Developmental cell 32, 707-18. PMID: 25805136

3. Panda, D. et al. (2015) mBio 6. PMID: 25852164

4. Baymaz, HI. et al. (2014) Proteomics 14, 2179-89. PMID: 24634419

5. Campagne, A. et al. (2019) Nature communications 10, 348. PMID: 30664650

6. Zhang, HL. et al. (2025) Nature chemical biology 21, 1531-1543. PMID: 40246981

Note

For research use only

图片

参考文献

产品说明书

Anti-FOXK1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-FOXK1 Polyclonal Antibody (HB309014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-FOXK1 Polyclonal Antibody (HB309014)

概述

图片

参考文献

产品说明书

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