Anti-UBE2H Polyclonal Antibody (HB330014)

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100ug

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概述

货号

HB330014

品牌

abinScience

描述

Anti-UBE2H Polyclonal Antibody (HB330014) is a rabbit polyclonal antibody detecting Ubiquitin-conjugating enzyme E2 H; 2.3.2.23; (E3-independent) E2 ubiquitin-conjugating enzyme H; 2.3.2.24; E2 ubiquitin-conjugating enzyme H; UbcH2; Ubiquitin carrier protein H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H; UBE2H in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human UBE2H (Met1-Leu183).

靶标

Ubiquitin-conjugating enzyme E2 H; 2.3.2.23; (E3-independent) E2 ubiquitin-conjugating enzyme H; 2.3.2.24; E2 ubiquitin-conjugating enzyme H; UbcH2; Ubiquitin carrier protein H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H; UBE2H

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P62256, P62257, Q32LN1

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Ubiquitin-conjugating enzyme E2 H (UBE2H) is a ~20 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.

1. Hoeller, D. et al. (2007) Molecular cell 26, 891-8. PMID: 17588522

2. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386

3. Kaiser, P. et al. (1994) The Journal of biological chemistry 269, 8797-802. PMID: 8132613

4. Lampert, F. et al. (2018) eLife 7. PMID: 29911972

Note

For research use only

图片

参考文献

产品说明书

Anti-UBE2H Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-UBE2H Polyclonal Antibody (HB330014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-UBE2H Polyclonal Antibody (HB330014)

概述

图片

参考文献

产品说明书

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