Anti-CRYZ Polyclonal Antibody (HB380014)

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规格：

50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HB380014

品牌

abinScience

描述

Anti-CRYZ Polyclonal Antibody (HB380014) is a rabbit polyclonal antibody detecting Quinone oxidoreductase; 1.6.5.5; NADPH:quinone reductase; Zeta-crystallin; CRYZ in ELISA, IHC, WB. Suitable for Human and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human CRYZ (Met1-Leu329).

靶标

Quinone oxidoreductase; 1.6.5.5; NADPH:quinone reductase; Zeta-crystallin; CRYZ

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q08257, O97764

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Zeta-crystallin (CRYZ) is a ~35 kDa protein. Multifunctional crystallin endowed with unrelated mRNA stabilization and enzymatic oxidoreductase activities. Binds (AU)-rich elements (ARE) in the 3'-UTR of target mRNA to enhance their stability. Upon metabolic acidosis, stabilizes the mRNA encoding SLC12A1 a cotransporter involved in transepithelial NH4(+) reabsorption in the medullary thick ascending limb in kidney. In response to acidosis, also participates to the adaptive increase in renal ammoniagenesis by stabilizing the mRNAs of enzymes involved in glutamine catabolism. By the same mechanism, it also regulates the expression of the antiapoptotic protein BCL2.

1. Fernández, MR. et al. (2007) Cellular and molecular life sciences : CMLS 64, 1419-27. PMID: 17497241

2. Lapucci, A. et al. (2010) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24, 1852-65. PMID: 20103721

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-CRYZ polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Raji cell lysate

参考文献

产品说明书

Anti-CRYZ Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CRYZ Polyclonal Antibody (HB380014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CRYZ Polyclonal Antibody (HB380014)

概述

图片

参考文献

产品说明书

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