Anti-SF3A3 Polyclonal Antibody (HB387014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HB387014

品牌

abinScience

描述

Anti-SF3A3 Polyclonal Antibody (HB387014) is a rabbit polyclonal antibody detecting Splicing factor 3A subunit 3; SF3a60; Spliceosome-associated protein 61; SAP 61; SF3A3; SAP61 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human SF3A3 (Met1-Leu501).

靶标

Splicing factor 3A subunit 3; SF3a60; Spliceosome-associated protein 61; SAP 61; SF3A3; SAP61

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q12874, Q9D554

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Splicing factor 3A subunit 3 (SF3A3) is a ~58 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A3 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes.

1. Das, R. et al. (2000) Molecular cell 5, 779-87. PMID: 10882114

2. Nesic, D. et al. (2001) Molecular and cellular biology 21, 6406-17. PMID: 11533230

3. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006

4. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310

5. Chiara, MD. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 6403-7. PMID: 8022796

6. Zhang, X. et al. (2018) Cell research 28, 307-322. PMID: 29360106

7. Zhan, X. et al. (2018) Cell research 28, 1129-1140. PMID: 30315277

Note

For research use only

图片

参考文献

产品说明书

Anti-SF3A3 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SF3A3 Polyclonal Antibody (HB387014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SF3A3 Polyclonal Antibody (HB387014)

概述

图片

参考文献

产品说明书

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