Anti-SMYD5 Polyclonal Antibody (HB389014)

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100ug

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概述

货号

HB389014

品牌

abinScience

描述

Anti-SMYD5 Polyclonal Antibody (HB389014) is a rabbit polyclonal antibody detecting Histone-lysine N-trimethyltransferase SMYD5; 2.1.1.372; Protein NN8-4AG; Retinoic acid-induced protein 15; SET and MYND domain-containing protein 5; [histone H4]-lysine20 N-trimethyltransferase SMYD5; SMYD5; RAI15 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human SMYD5 (Met1-Glu384).

靶标

Histone-lysine N-trimethyltransferase SMYD5; 2.1.1.372; Protein NN8-4AG; Retinoic acid-induced protein 15; SET and MYND domain-containing protein 5; [histone H4]-lysine20 N-trimethyltransferase SMYD5; SMYD5; RAI15

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q6GMV2, Q3TYX3

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Protein-lysine N-trimethyltransferase SMYD5 is a ~47 kDa protein. Protein-lysine N-trimethyltransferase that specifically catalyzes trimethylation of 'Lys-22' of the RPL40/eL40 subunit of the 60S ribosome, thereby promoting translation elongation and protein synthesis. May also act as a histone methyltransferase in the context of histone octamers, but not on nucleosome substrates: trimethylates 'Lys-36' of histone H3 and 'Lys-20' of histone H4 to form H3K36me3 and H4K20me3, respectively. The histone methyltransferase activity, which is independent of its SET domain, is however unsure in vivo. In association with the NCoR corepressor complex, involved in the repression of toll-like receptor 4 (TLR4)-target inflammatory genes in macrophages, possibly by catalyzing the formation of H4K20me3 at the gene promoters. Plays an important role in embryonic stem (ES) cell self-renewal and differentiation.

1. Park, J. et al. (2024) Nature 632, 656-663. PMID: 39048817

2. Miao, B. et al. (2024) Cell research 34, 648-660. PMID: 39103523

3. Kidder, BL. et al. (2017) Cancer research 77, 6729-6745. PMID: 28951459

Note

For research use only

图片

参考文献

产品说明书

Anti-SMYD5 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SMYD5 Polyclonal Antibody (HB389014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SMYD5 Polyclonal Antibody (HB389014)

概述

图片

参考文献

产品说明书

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