Anti-RHO Polyclonal Antibody (HY021014)

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概述
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参考文献
产品说明书

概述

货号

HY021014

品牌

abinScience

描述

Anti-RHO Polyclonal Antibody (HY021014) is a rabbit polyclonal antibody detecting Rhodopsin; Opsin-2; RHO; OPN2 in ELISA, IHC, WB. Suitable for Human, Dog, Pig, Cat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human RHO (Asn2-Gln36&Thr97-Cys110&Gly174-Ser202).

靶标

Rhodopsin; Opsin-2; RHO; OPN2

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P08100, P32308, O18766, Q95KU1, P15409, P02700, P51489, P02699, P29403, P28683, P49912

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Rhodopsin is a ~38 kDa protein. Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling.

1. Sieving, PA. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 880-4. PMID: 7846071

2. Rao, VR. et al. (1994) Nature 367, 639-42. PMID: 8107847

3. Noorwez, SM. et al. (2003) The Journal of biological chemistry 278, 14442-14450. PMID: 12566452

4. Dryja, TP. et al. (1990) The New England journal of medicine 323, 1302-7. PMID: 2215617

5. Kang, Y. et al. (2015) Nature 523, 561-7. PMID: 26200343

6. He, Y. et al. (2017) Cell research 27, 728-747. PMID: 28524165

7. Zhou, XE. et al. (2017) Cell 170, 457-469.e13. PMID: 28753425

Note

For research use only

图片

参考文献

产品说明书

Anti-RHO Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-RHO Polyclonal Antibody (HY021014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-RHO Polyclonal Antibody (HY021014)

概述

图片

参考文献

产品说明书

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