Anti-FHIT Polyclonal Antibody (HB415014)

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100ug

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概述
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产品说明书

概述

货号

HB415014

品牌

abinScience

描述

Anti-FHIT Polyclonal Antibody (HB415014) is a rabbit polyclonal antibody detecting P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein; FHIT in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human FHIT (Met1-Gln147).

靶标

Bis(5'-adenosyl)-triphosphatase; 3.6.1.29; AP3A hydrolase; AP3Aase; Adenosine 5'-monophosphoramidase FHIT; 3.9.1.-; Adenylylsulfatase; 3.6.2.1; Adenylylsulfate-ammonia adenylyltransferase; 2.7.7.51; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein; FHIT

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P49789, O89106, Q1KZG4, Q9JIX3

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Bis(5'-adenosyl)-triphosphatase (FHIT) is a ~16 kDa protein. Possesses dinucleoside triphosphate hydrolase activity. Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Exhibits adenylylsulfatase activity, hydrolyzing adenosine 5'-phosphosulfate to yield AMP and sulfate. Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2.

1. Trapasso, F. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 1592-7. PMID: 12574506

2. Huang, K. et al. (2004) Biochemistry 43, 7637-42. PMID: 15182206

3. Barnes, LD. et al. (1996) Biochemistry 35, 11529-35. PMID: 8794732

4. Lima, CD. et al. (1997) Science (New York, N.Y.) 278, 286-90. PMID: 9323207

5. Brenner, C. et al. (1997) Protein engineering 10, 1461-3. PMID: 9543008

6. Pace, HC. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 5484-9. PMID: 9576908

9. Weiske, J. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 20344-9. PMID: 18077326

Note

For research use only

图片

参考文献

产品说明书

Anti-FHIT Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-FHIT Polyclonal Antibody (HB415014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-FHIT Polyclonal Antibody (HB415014)

概述

图片

参考文献

产品说明书

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