Anti-STARD3 Polyclonal Antibody (HB422014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HB422014

品牌

abinScience

描述

Anti-STARD3 Polyclonal Antibody (HB422014) is a rabbit polyclonal antibody detecting StAR-related lipid transfer protein 3; Metastatic lymph node gene 64 protein; MLN 64; Protein CAB1; START domain-containing protein 3; StARD3; STARD3; CAB1; MLN64 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human STARD3 (Ser232-Ala445).

靶标

StAR-related lipid transfer protein 3; Metastatic lymph node gene 64 protein; MLN 64; Protein CAB1; START domain-containing protein 3; StARD3; STARD3; CAB1; MLN64

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q14849, Q61542

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

StAR-related lipid transfer protein 3 (STARD3) is a ~50 kDa protein. Sterol-binding protein that mediates cholesterol transport from the endoplasmic reticulum to endosomes. The sterol transport mechanism is triggered by phosphorylation of FFAT motif that leads to membrane tethering between the endoplasmic reticulum and late endosomes via interaction with VAPA and VAPB. Acts as a lipid transfer protein that redirects sterol to the endosome at the expense of the cell membrane and favors membrane formation inside endosomes. May also mediate cholesterol transport between other membranes, such as mitochondria membrane or cell membrane. However, such results need additional experimental evidences; probably mainly mediates cholesterol transport from the endoplasmic reticulum to endosomes.

1. Alpy, F. et al. (2001) The Journal of biological chemistry 276, 4261-9. PMID: 11053434

2. Hölttä-Vuori, M. et al. (2005) Molecular biology of the cell 16, 3873-86. PMID: 15930133

3. Liapis, A. et al. (2012) PloS one 7, e34424. PMID: 22514632

4. Wilhelm, LP. et al. (2017) The EMBO journal 36, 1412-1433. PMID: 28377464

5. Di Mattia, T. et al. (2020) The EMBO journal 39, e104369. PMID: 33124732

6. Alpy, F. et al. (2013) Journal of cell science 126, 5500-12. PMID: 24105263

7. Zhang, M. et al. (2002) The Journal of biological chemistry 277, 33300-10. PMID: 12070139

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-STARD3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: MCF-7 cell lysate
Lane 2: Mouse brain lysate
Lane 3: Mouse lung lysate
Lane 4: Mouse heart lysate
Lane 5: Rat brain lysate
Lane 6: Rat heart lysate

参考文献

产品说明书

Anti-STARD3 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-STARD3 Polyclonal Antibody (HB422014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-STARD3 Polyclonal Antibody (HB422014)

概述

图片

参考文献

产品说明书

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