Anti-ADPRS Polyclonal Antibody (HB443014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HB443014

品牌

abinScience

描述

Anti-ADPRS Polyclonal Antibody (HB443014) is a rabbit polyclonal antibody detecting ADP-ribosylhydrolase ARH3; ADP-ribose glycohydrolase ARH3; ADP-ribosylhydrolase 3; O-acetyl-ADP-ribose deacetylase ARH3; 3.5.1.-; Poly(ADP-ribose) glycohydrolase ARH3; 3.2.1.143; [Protein ADP-ribosylarginine] hydrolase-like protein 2; [Protein ADP-ribosylserine] hydrolase; 3.2.2.-; ADPRS; ADPRHL2; ARH3 in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human ADPRS (Ser19-Ser363).

靶标

ADP-ribosylhydrolase ARH3; ADP-ribose glycohydrolase ARH3; ADP-ribosylhydrolase 3; O-acetyl-ADP-ribose deacetylase ARH3; 3.5.1.-; Poly(ADP-ribose) glycohydrolase ARH3; 3.2.1.143; [Protein ADP-ribosylarginine] hydrolase-like protein 2; [Protein ADP-ribosylserine] hydrolase; 3.2.2.-; ADPRS; ADPRHL2; ARH3

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q9NX46, Q3SYV9, Q8CG72

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

ADP-ribosylhydrolase ARH3 (ADPRS) is a ~38 kDa protein. ADP-ribosylhydrolase that preferentially hydrolyzes the scissile alpha-O-linkage attached to the anomeric C1'' position of ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on serine and threonine, free poly(ADP-ribose) and O-acetyl-ADP-D-ribose. Specifically acts as a serine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to serine residues on proteins, thereby playing a key role in DNA damage response. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Does not hydrolyze ADP-ribosyl-arginine, -cysteine, -diphthamide, or -asparagine bonds. Also able to degrade protein free poly(ADP-ribose), which is synthesized in response to DNA damage: free poly(ADP-ribose) acts as a potent cell death signal and its degradation by ADPRHL2 protects cells from poly(ADP-ribose)-dependent cell death, a process named parthanatos.

1. Kasamatsu, A. et al. (2011) The Journal of biological chemistry 286, 21110-7. PMID: 21498885

2. Pourfarjam, Y. et al. (2018) The Journal of biological chemistry 293, 12350-12359. PMID: 29907568

3. Wang, M. et al. (2018) The Journal of biological chemistry 293, 14470-14480. PMID: 30045870

4. Danhauser, K. et al. (2018) American journal of human genetics 103, 817-825. PMID: 30401461

5. Mashimo, M. et al. (2019) JCI insight 4. PMID: 30830864

6. Bonfiglio, JJ. et al. (2020) Cell 183, 1086-1102.e23. PMID: 33186521

7. Voorneveld, J. et al. (2021) Chemistry (Weinheim an der Bergstrasse, Germany) 27, 10621-10627. PMID: 33769608

8. Pourfarjam, Y. et al. (2021) The Journal of biological chemistry 296, 100692. PMID: 33894202

9. Prokhorova, E. et al. (2021) Molecular cell 81, 2640-2655.e8. PMID: 34019811

10. Rack, JGM. et al. (2021) Nature communications 12, 4581. PMID: 34321462

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-ADPRS polyclonal antibody at 1ug/mL on various samples.
Lane 1: Rat brain lysate
Lane 2: Rat heart lysate

参考文献

产品说明书

Anti-ADPRS Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ADPRS Polyclonal Antibody (HB443014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ADPRS Polyclonal Antibody (HB443014)

概述

图片

参考文献

产品说明书

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