Anti-SNRPD3 Polyclonal Antibody (HB460014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HB460014

品牌

abinScience

描述

Anti-SNRPD3 Polyclonal Antibody (HB460014) is a rabbit polyclonal antibody detecting Small nuclear ribonucleoprotein Sm D3; Sm-D3; snRNP core protein D3; SNRPD3 in ELISA, IHC, WB. Suitable for Human, Mouse, and Frog.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human SNRPD3 (Met1-Arg126).

靶标

Small nuclear ribonucleoprotein Sm D3; Sm-D3; snRNP core protein D3; SNRPD3

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P62318, P62320, P62323

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Small nuclear ribonucleoprotein Sm D3 (SNRPD3) is a ~13 kDa protein. Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. As part of the U7 snRNP it is involved in histone pre-mRNA 3'-end processing.

1. Jurica, MS. et al. (2002) RNA (New York, N.Y.) 8, 426-39. PMID: 11991638

2. Chari, A. et al. (2008) Cell 135, 497-509. PMID: 18984161

3. Pomeranz Krummel, DA. et al. (2009) Nature 458, 475-80. PMID: 19325628

4. Kondo, Y. et al. (2015) eLife 4. PMID: 25555158

5. Agafonov, DE. et al. (2016) Science (New York, N.Y.) 351, 1416-20. PMID: 26912367

6. Bertram, K. et al. (2017) Nature 542, 318-323. PMID: 28076346

7. Zhang, X. et al. (2017) Cell 169, 918-929.e14. PMID: 28502770

8. Bertram, K. et al. (2017) Cell 170, 701-713.e11. PMID: 28781166

9. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-SNRPD3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: MCF-7 cell lysate
Lane 2: HT-29 cell lysate
Lane 3: 293T cell lysate
Lane 4: Jurkat cell lysate
Lane 5: Caco2 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Rat brain lysate
Lane 8: Rat liver lysate

参考文献

产品说明书

Anti-SNRPD3 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SNRPD3 Polyclonal Antibody (HB460014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SNRPD3 Polyclonal Antibody (HB460014)

概述

图片

参考文献

产品说明书

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