Anti-PGAM5 Polyclonal Antibody (HB500014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HB500014

品牌

abinScience

描述

Anti-PGAM5 Polyclonal Antibody (HB500014) is a rabbit polyclonal antibody detecting Serine/threonine-protein phosphatase PGAM5 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human PGAM5 (Ala94-Ser289).

靶标

Serine/threonine-protein phosphatase PGAM5, mitochondrial; 3.1.3.16; Bcl-XL-binding protein v68; Phosphoglycerate mutase family member 5; PGAM5

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q96HS1, Q8BX10, Q562B5

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Serine/threonine-protein phosphatase PGAM5, mitochondrial is a ~32 kDa protein. Mitochondrial serine/threonine phosphatase that dephosphorylates various substrates and thus plays a role in different biological processes including cellular senescence or mitophagy. Modulates cellular senescence by regulating mitochondrial dynamics. Mechanistically, participates in mitochondrial fission through dephosphorylating DNM1L/DRP1. Additionally, dephosphorylates MFN2 in a stress-sensitive manner and consequently protects it from ubiquitination and degradation to promote mitochondrial network formation. Regulates mitophagy independent of PARKIN by interacting with and dephosphorylating FUNDC1, which interacts with LC3.

1. Chen, G. et al. (2014) Molecular cell 54, 362-77. PMID: 24746696

2. Yu, B. et al. (2020) Nature communications 11, 2549. PMID: 32439975

3. Nag, S. et al. (2023) Cell reports 42, 112895. PMID: 37498743

4. Lo, SC. et al. (2008) Experimental cell research 314, 1789-803. PMID: 18387606

5. Wang, Z. et al. (2012) Cell 148, 228-43. PMID: 22265414

Note

For research use only

图片

参考文献

产品说明书

Anti-PGAM5 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PGAM5 Polyclonal Antibody (HB500014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PGAM5 Polyclonal Antibody (HB500014)

概述

图片

参考文献

产品说明书

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