Anti-NOP2 Polyclonal Antibody (HB503014)

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50ug
100ug

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概述
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概述

货号

HB503014

品牌

abinScience

描述

Anti-NOP2 Polyclonal Antibody (HB503014) is a rabbit polyclonal antibody detecting Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase; 2.1.1.-; Nucleolar protein 1; Nucleolar protein 2 homolog; Proliferating-cell nucleolar antigen p120; Proliferation-associated nucleolar protein p120; NOP2; NOL1; NSUN1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human NOP2 (Glu217-Ser588).

靶标

Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase; 2.1.1.-; Nucleolar protein 1; Nucleolar protein 2 homolog; Proliferating-cell nucleolar antigen p120; Proliferation-associated nucleolar protein p120; NOP2; NOL1; NSUN1

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P46087, Q922K7

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

28S rRNA (cytosine(4447)-C(5))-methyltransferase (NOP2) is a ~89 kDa protein. S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 4447 in 28S rRNA. Required for efficient rRNA processing and 60S ribosomal subunit biogenesis. Regulates pre-rRNA processing through non-catalytic complex formation with box C/D snoRNAs and facilitates the recruitment of U3 and U8 snoRNAs to pre-90S ribosomal particles and their stable assembly into snoRNP complexes. May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation.

1. Bourgeois, G. et al. (2015) PloS one 10, e0133321. PMID: 26196125

2. Sloan, KE. et al. (2013) Cell reports 5, 237-47. PMID: 24120868

3. Liao, H. et al. (2022) Nucleic acids research 50, 10695-10716. PMID: 36161484

Note

For research use only

图片

参考文献

产品说明书

Anti-NOP2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-NOP2 Polyclonal Antibody (HB503014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-NOP2 Polyclonal Antibody (HB503014)

概述

图片

参考文献

产品说明书

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