Anti-PHF2 Polyclonal Antibody (HB551014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HB551014

品牌

abinScience

描述

Anti-PHF2 Polyclonal Antibody (HB551014) is a rabbit polyclonal antibody detecting Lysine-specific demethylase PHF2; 1.14.11.-; GRC5; PHD finger protein 2; PHF2; CENP-35; KIAA0662 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human PHF2 (Gln83-Arg450).

靶标

Lysine-specific demethylase PHF2; 1.14.11.-; GRC5; PHD finger protein 2; PHF2; CENP-35; KIAA0662

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

O75151, Q9WTU0

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Lysine-specific demethylase PHF2 is a ~120 kDa protein. Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.

1. Wen, H. et al. (2010) The Journal of biological chemistry 285, 9322-9326. PMID: 20129925

2. Horton, JR. et al. (2011) Journal of molecular biology 406, 1-8. PMID: 21167174

3. Baba, A. et al. (2011) Nature cell biology 13, 668-75. PMID: 21532585

Note

For research use only

图片

参考文献

产品说明书

Anti-PHF2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PHF2 Polyclonal Antibody (HB551014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PHF2 Polyclonal Antibody (HB551014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价