Anti-CHMP4B Polyclonal Antibody (HB565014)

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100ug

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概述
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概述

货号

HB565014

品牌

abinScience

描述

Anti-CHMP4B Polyclonal Antibody (HB565014) is a rabbit polyclonal antibody detecting Charged multivesicular body protein 4b; Chromatin-modifying protein 4b; CHMP4b; SNF7 homolog associated with Alix 1; SNF7-2; hSnf7-2; Vacuolar protein sorting-associated protein 32-2; Vps32-2; hVps32-2; CHMP4B; C20orf178; SHAX1 in ELISA, IHC, WB. Suitable for Human, Mouse, Chicken, and Frog.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human CHMP4B (Asp121-Met224).

靶标

Charged multivesicular body protein 4b; Chromatin-modifying protein 4b; CHMP4b; SNF7 homolog associated with Alix 1; SNF7-2; hSnf7-2; Vacuolar protein sorting-associated protein 32-2; Vps32-2; hVps32-2; CHMP4B; C20orf178; SHAX1

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q9H444, Q9D8B3, P59074, Q5ZHP5, Q5XGW6

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Charged multivesicular body protein 4b (CHMP4B) is a ~24 kDa protein. Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis.

1. Katoh, K. et al. (2003) The Journal of biological chemistry 278, 39104-13. PMID: 12860994

2. Hanson, PI. et al. (2008) The Journal of cell biology 180, 389-402. PMID: 18209100

3. Guizetti, J. et al. (2011) Science (New York, N.Y.) 331, 1616-20. PMID: 21310966

4. Vietri, M. et al. (2015) Nature 522, 231-5. PMID: 26040712

5. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413

6. Baeumers, M. et al. (2020) BMC biology 18, 200. PMID: 33349255

Note

For research use only

图片

参考文献

产品说明书

Anti-CHMP4B Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CHMP4B Polyclonal Antibody (HB565014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CHMP4B Polyclonal Antibody (HB565014)

概述

图片

参考文献

产品说明书

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