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Anti-EIF1AX Polyclonal Antibody (HB566014)

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概述
货号HB566014
品牌abinScience
描述
Anti-EIF1AX Polyclonal Antibody (HB566014) is a rabbit polyclonal antibody detecting X-chromosomal; eIF-1A X isoform; eIF1A X isoform; Eukaryotic translation initiation factor 4C; eIF-4C; EIF1AX; EIF1A; EIF4C in ELISA, IHC, WB. Suitable for Mouse, Human, Rabbit, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant human EIF1AX (Asn19-Ile144).
靶标Eukaryotic translation initiation factor 1A, X-chromosomal; eIF-1A X isoform; eIF1A X isoform; Eukaryotic translation initiation factor 4C; eIF-4C; EIF1AX; EIF1A; EIF4C
内毒素水平Please contact with the lab for this information.
纯化方式Purified by antigen affinity column.
Accession号Q8BMJ3, P47813, O14602, P47814, Q6VV72, Q60872
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Eukaryotic translation initiation factor 1A, X-chromosomal (EIF1AX) is a ~16 kDa protein. Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. This protein enhances formation of the cap-proximal complex. Together with EIF1, facilitates scanning, start codon recognition, promotion of the assembly of 48S complex at the initiation codon (43S PIC becomes 48S PIC after the start codon is reached), and dissociation of aberrant complexes. After start codon location, together with EIF5B orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after 80S initiation complex formation, just after GTP hydrolysis by EIF5B, and before release of EIF5B.

NoteFor research use only
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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