Anti-MRAS Polyclonal Antibody (HB573014)

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100ug

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概述
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产品说明书

概述

货号

HB573014

品牌

abinScience

描述

Anti-MRAS Polyclonal Antibody (HB573014) is a rabbit polyclonal antibody detecting Ras-related protein M-Ras; 3.6.5.2; Ras-related protein R-Ras3; MRAS; RRAS3 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human MRAS (Met1-Gln204).

靶标

Ras-related protein M-Ras; 3.6.5.2; Ras-related protein R-Ras3; MRAS; RRAS3

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

O14807, O08989, P97538

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Ras-related protein M-Ras (MRAS) is a ~23 kDa protein. Signal transducer in the Ras-MAPK signaling pathway that regulates cell proliferation and survival. Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation. The formation of the SMP complex only occurs when MRAS is GTP-bound. MRAS has low intrinsic GTPase activity and may require additional factors for activation. The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities.

1. Rodriguez-Viciana, P. et al. (2006) Molecular cell 22, 217-30. PMID: 16630891

2. Higgins, EM. et al. (2017) JCI insight 2, e91225. PMID: 28289718

3. Liau, NPD. et al. (2022) Nature 609, 400-407. PMID: 35768504

4. Hauseman, ZJ. et al. (2022) Nature 609, 416-423. PMID: 35830882

5. Kwon, JJ. et al. (2022) Nature 609, 408-415. PMID: 35831509

6. Bonsor, DA. et al. (2022) Nature structural & molecular biology 29, 966-977. PMID: 36175670

7. Bernal Astrain, G. et al. (2025) Nature communications 16, 647. PMID: 39809765

Note

For research use only

图片

参考文献

产品说明书

Anti-MRAS Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MRAS Polyclonal Antibody (HB573014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MRAS Polyclonal Antibody (HB573014)

概述

图片

参考文献

产品说明书

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