Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Ubiquitin carboxyl-terminal hydrolase 12 (USP12) is a ~42 kDa protein. Deubiquitinating enzyme that plays various roles in the regulation of the immune response and inflammation. During TCR engagement and activation, translocates into the cytoplasm and deubiquitinates its substrates LAT and TRAT1 and prevents their lysosome-dependent degradation to stabilize the TCR signaling complex at the plasma membrane. Plays an essential role in the selective LPS-induced macrophage response through the activation of NF-kappa-B pathway. In addition, promotes that antiviral immune response through targeting DNA sensor IFI16 to inhibit its proteasome-dependent degradation. Participates in the interferon signaling pathway and antiviral response independently of its deubiquitinase activity by maintaining nuclear phosphorylated STAT1 levels via inhibition of its CREBBP-mediated acetylation and subsequent dephosphorylation.
1. Cohn, MA. et al. (2009) The Journal of biological chemistry 284, 5343-51. PMID: 19075014
2. Li, H. et al. (2016) Molecular cell 63, 249-260. PMID: 27373336
3. Jahan, AS. et al. (2016) Proceedings of the National Academy of Sciences of the United States of America 113, E705-14. PMID: 26811477
4. Nayak, TKS. et al. (2017) Biochemical and biophysical research communications 483, 69-74. PMID: 28063927
5. Fu, Y. et al. (2023) PLoS pathogens 19, e1011480. PMID: 37410794
6. Liu, J. et al. (2020) PLoS pathogens 16, e1008215. PMID: 31899788
7. Choi, HS. et al. (2022) International journal of molecular sciences 23. PMID: 36361894
8. Moretti, J. et al. (2012) The Journal of biological chemistry 287, 29429-41. PMID: 22778262
9. Aron, R. et al. (2018) Nature communications 9, 3191. PMID: 30266909
10. Gangula, NR. et al. (2013) The Journal of biological chemistry 288, 34545-54. PMID: 24145035
