Anti-Human ZBED1 Polyclonal Antibody (HB594014)

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50ug
100ug

数量：

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概述
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产品说明书

概述

货号

HB594014

品牌

abinScience

描述

Anti-Human ZBED1 Polyclonal Antibody (HB594014) is a rabbit polyclonal antibody detecting E3 SUMO-protein ligase ZBED1; 2.3.2.-; DNA replication-related element-binding factor; Putative Ac-like transposable element; Zinc finger BED domain-containing protein 1; dREF homolog; ZBED1; ALTE; DREF; hDREF; KIAA0785; TRAMP in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human ZBED1 (Asp119-Cys290).

靶标

E3 SUMO-protein ligase ZBED1; 2.3.2.-; DNA replication-related element-binding factor; Putative Ac-like transposable element; Zinc finger BED domain-containing protein 1; dREF homolog; ZBED1; ALTE; DREF; hDREF; KIAA0785; TRAMP

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

O96006

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

E3 SUMO-protein ligase ZBED1 is a ~78 kDa protein. Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase which sumoylates CHD3/Mi2-alpha, causing its release from DNA. This results in suppression of CHD3/Mi2-alpha transcription repression, increased recruitment of RNA polymerase II to gene promoters and positive regulation of transcription including H1-5 and ribosomal proteins such as: RPS6, RPL10A, and RPL12. The resulting increased transcriptional activity drives cell proliferation. Binds to 5'-TGTCG[CT]GA[CT]A-3' consensus sequences in gene promoters of ribosomal proteins.

1. Yamashita, D. et al. (2016) The Journal of biological chemistry 291, 11619-34. PMID: 27068747

2. Ohshima, N. et al. (2003) The Journal of biological chemistry 278, 22928-38. PMID: 12663651

3. Yamashita, D. et al. (2007) The Journal of biological chemistry 282, 7563-75. PMID: 17209048

4. Yamashita, D. et al. (2007) Molecular and cellular biology 27, 2003-13. PMID: 17220279

Note

For research use only

图片

参考文献

产品说明书

Anti-Human ZBED1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ZBED1 Polyclonal Antibody (HB594014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ZBED1 Polyclonal Antibody (HB594014)

概述

图片

参考文献

产品说明书

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