Anti-Mouse MLKL Polyclonal Antibody (MV568014)

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概述

货号

MV568014

品牌

abinScience

描述

Anti-Mouse MLKL Polyclonal Antibody (MV568014) is a rabbit polyclonal antibody detecting Mixed lineage kinase domain-like protein; Mlkl in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant mouse MLKL (Asp180-Val472).

靶标

Mixed lineage kinase domain-like protein; Mlkl

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q9D2Y4

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Mixed lineage kinase domain-like protein (MLKL) is a ~54 kDa protein. Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Does not have protein kinase activity. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function.

1. Wu, J. et al. (2013) Cell research 23, 994-1006. PMID: 23835476

2. Murphy, JM. et al. (2013) Immunity 39, 443-53. PMID: 24012422

3. Kaiser, WJ. et al. (2013) The Journal of biological chemistry 288, 31268-79. PMID: 24019532

4. Nogusa, S. et al. (2016) Cell host & microbe 20, 13-24. PMID: 27321907

5. Zhang, T. et al. (2020) Cell 180, 1115-1129.e13. PMID: 32200799

6. Jiao, H. et al. (2020) Nature 580, 391-395. PMID: 32296175

Note

For research use only

图片

参考文献

产品说明书

Anti-Mouse MLKL Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse MLKL Polyclonal Antibody (MV568014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse MLKL Polyclonal Antibody (MV568014)

概述

图片

参考文献

产品说明书

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