Anti-Mouse Ki67/MKI67 Polyclonal Antibody (MW621014)

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50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

MW621014

品牌

abinScience

描述

Anti-Mouse Ki67/MKI67 Polyclonal Antibody (MW621014) is a rabbit polyclonal antibody detecting Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67 homolog; Antigen Ki67 homolog; Mki67 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant mouse Ki67/MKI67 (Met1-Pro120).

靶标

Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67 homolog; Antigen Ki67 homolog; Mki67

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

E9PVX6

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Proliferation marker protein Ki-67 (Ki67/MKI67) is a ~350 kDa protein. Protein that associates with the surface of mitotic chromosomes and acts both as a chromosome repellent during early mitosis and chromosome attractant during late mitosis. Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. During early mitosis, relocalizes from nucleoli to the chromosome surface where it forms extended brush structures that cover a substantial fraction of the chromosome surface. The MKI67 brush structure prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. During mitotic anaphase, the MKI67 brush structure collapses and MKI67 switches from a chromosome repellent to a chromosome attractant to promote chromosome clustering and facilitate the exclusion of large cytoplasmic particles from the future nuclear space.

1. Cuylen, S. et al. (2016) Nature 535, 308-12. PMID: 27362226

2. Sobecki, M. et al. (2016) eLife 5, e13722. PMID: 26949251

Note

For research use only

图片

参考文献

产品说明书

Anti-Mouse Ki67/MKI67 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse Ki67/MKI67 Polyclonal Antibody (MW621014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse Ki67/MKI67 Polyclonal Antibody (MW621014)

概述

图片

参考文献

产品说明书

相关产品推荐

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