Anti-Mouse ANG Polyclonal Antibody (MY052014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

MY052014

品牌

abinScience

描述

Anti-Mouse ANG Polyclonal Antibody (MY052014) is a rabbit polyclonal antibody detecting Angiogenin; 3.1.27.-; Angiogenin-1; Ribonuclease 5; RNase 5; Ang; Ang1; Rnase5; Rnase5a in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant mouse ANG (Gln25-Leu145).

靶标

Angiogenin; 3.1.27.-; Angiogenin-1; Ribonuclease 5; RNase 5; Ang; Ang1; Rnase5; Rnase5a

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P21570

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Angiogenin is a ~16 kDa protein. Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context. Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus. Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs): specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation. tiRNas also prevent formation of apoptosome, thereby promoting cell survival. Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36.

1. Fu, H. et al. (2009) FEBS letters 583, 437-42. PMID: 19114040

2. Goncalves, KA. et al. (2016) Cell 166, 894-906. PMID: 27518564

3. Bai, R. et al. (2020) The EMBO journal 39, e103325. PMID: 32510170

4. Liu, X. et al. (2021) Nature communications 12, 1832. PMID: 33758201

5. Yue, T. et al. (2021) Science (New York, N.Y.) 372. PMID: 33986151

6. Yu, W. et al. (2017) Cell 171, 849-864.e25. PMID: 29100074

7. Saikia, M. et al. (2014) Molecular and cellular biology 34, 2450-63. PMID: 24752898

Note

For research use only

图片

参考文献

产品说明书

Anti-Mouse ANG Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse ANG Polyclonal Antibody (MY052014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse ANG Polyclonal Antibody (MY052014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价