Anti-IFI30 Polyclonal Antibody (MY158014)

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100ug

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概述
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产品说明书

概述

货号

MY158014

品牌

abinScience

描述

Anti-IFI30 Polyclonal Antibody (MY158014) is a rabbit polyclonal antibody detecting Gamma-interferon-inducible lysosomal thiol reductase; 1.8.-.-; Gamma-interferon-inducible protein IP-30; Lysosomal thiol reductase IP30; Ifi30; Gilt; Ip30 in ELISA, IHC, WB. Suitable for Rat and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant mouse IFI30 (Val59-Lys248).

靶标

Gamma-interferon-inducible lysosomal thiol reductase; 1.8.-.-; Gamma-interferon-inducible protein IP-30; Lysosomal thiol reductase IP30; Ifi30; Gilt; Ip30

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q9ESY9, Q499T2

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Gamma-interferon-inducible lysosomal thiol reductase (IFI30) is a ~27 kDa protein. Lysosomal thiol reductase that catalyzes protein disulfide bonds reduction. Plays an important role in antigen processing and presentation, of namely both major histocompatibility complex (MHC) class I- and class II-restricted antigen by reducing the disulfide bonds of endocytosed proteins and facilitating their unfolding and optimal degradation. Shares with thioredoxin a reduction mechanism in which the N-terminal cysteine thiol group in the CXXC active site motif initiates a nucleophilic attack on the substrate disulfide bond,resulting in the formation of a mixed disulfide-linked enzyme-substrate intermediate (Probable). Subsequent intramolecular attack by the second cysteine thiol group enables the release of the reduced substrate and oxidized enzyme (Probable). Lysosomal cysteine is a physiological reducing agent that is capable of reducing IFI30, so that it can catalyzes the next reaction.

1. Maric, M. et al. (2001) Science (New York, N.Y.) 294, 1361-5. PMID: 11701933

2. Singh, R. et al. (2010) Science (New York, N.Y.) 328, 1394-8. PMID: 20538950

Note

For research use only

图片

参考文献

产品说明书

Anti-IFI30 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-IFI30 Polyclonal Antibody (MY158014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-IFI30 Polyclonal Antibody (MY158014)

概述

图片

参考文献

产品说明书

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