Anti-Rice CENH3 Polyclonal Antibody (PR109024)

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概述
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参考文献
产品说明书

概述

货号

PR109024

品牌

abinScience

描述

Anti-Rice CENH3 Polyclonal Antibody (PR109024) is a rabbit polyclonal antibody detecting Histone H3-like centromeric protein CENH3; Centromeric histone CENH3; Centromeric histone H3; CENH3; Os05g0489800; LOC_Os05g41080; OJ1119_H02.19; OsJ_19013 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Oryza sativa (Rice)

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant rice CENH3 (Gly71-Trp164).

靶标

Histone H3-like centromeric protein CENH3; Centromeric histone CENH3; Centromeric histone H3; CENH3; Os05g0489800; LOC_Os05g41080; OJ1119_H02.19; OsJ_19013

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q6T367

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Histone H3-like centromeric protein CENH3 is a ~18 kDa protein. Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation (Probable).

1. Nagaki, K. et al. (2004) Nature genetics 36, 138-45. PMID: 14716315

2. Yan, H. et al. (2006) The Plant cell 18, 2123-33. PMID: 16877494

Note

For research use only

图片

Western blot

Lane 1: HCT116 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HT-29 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: Raji cell lysate
Lane 6: Hela cell lysate
Lane 7: A375 cell lysate
Lane 8: A431 cell lysate
Lane 9: K562 cell lysate

参考文献

产品说明书

Anti-Rice CENH3 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Rice CENH3 Polyclonal Antibody (PR109024)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Rice CENH3 Polyclonal Antibody (PR109024)

概述

图片

参考文献

产品说明书

相关产品推荐

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