Anti-ARL8B Polyclonal Antibody (HC083014) is a rabbit polyclonal antibody detecting ARL8B in ELISA, IHC, WB. Suitable for Human, Rat, Mouse, Bovine, and Zebrafish.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human, Mouse, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
克隆类型
Polyclonal
同种型
IgG
免疫原
E. coli - derived recombinant Human ARL8B (Met1-Ser186).
靶标
ADP-ribosylation factor-like protein 8B, EC:3.6.5.2, ADP-ribosylation factor-like protein 10C, Novel small G protein indispensable for equal chromosome segregation 1, ARL8B, ARL10C, GIE1
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
ADP-ribosylation factor-like protein 8B is a ~21 kDa protein. Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of lysosomal positioning which is important for nutrient sensing, natural killer cell-mediated cytotoxicity and antigen presentation. Along with its effectors, orchestrates lysosomal transport and fusion. Localizes specifically to lysosomal membranes and mediates anterograde lysosomal motility by recruiting PLEKHM2, which in turn recruits the motor protein kinesin-1 on lysosomes. Required for lysosomal and cytolytic granule exocytosis.
1. Okai, T. et al. (2004) Journal of cell science 117, 4705-15. PMID: 15331635
2. Hofmann, I. et al. (2006) Journal of cell science 119, 1494-503. PMID: 16537643
3. Bagshaw, RD. et al. (2006) Biochemical and biophysical research communications 344, 1186-91. PMID: 16650381
4. Pu, J. et al. (2015) Developmental cell 33, 176-88. PMID: 25898167
5. Deshar, R. et al. (2016) The FEBS journal 283, 4583-4599. PMID: 27808481
6. Marwaha, R. et al. (2017) The Journal of cell biology 216, 1051-1070. PMID: 28325809
7. Rosa-Ferreira, C. et al. (2011) Developmental cell 21, 1171-8. PMID: 22172677
8. Tuli, A. et al. (2013) Molecular biology of the cell 24, 3721-35. PMID: 24088571
9. Michelet, X. et al. (2018) Journal of immunology (Baltimore, Md. : 1950) 200, 3160-3169. PMID: 29592961
10. Garg, S. et al. (2011) Immunity 35, 182-93. PMID: 21802320
Note
For research use only
图片
Western blot
Western blot analysis was performed using anti-ARF1 polyclonal antibody at 1μg/ml on various samples. Lane 1: Hela cell lysate Lane 2: SKOV3 cell lysate Lane 3: DU145 cell lysate Lane 4: HepG2 cell lysate Lane 5: Lncap cell lysate Lane 6: 293T cell lysate Lane 7: Mouse brain lysate Lane 8: Mouse spleen lysate Lane 9: Mouse lung lysate Lane 10: Rat brain lysate
