Anti-HPV18 E7/Protein E7 Polyclonal Antibody (VK556024)

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100ug

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概述
图片
参考文献
产品说明书

概述

货号

VK556024

品牌

abinScience

描述

Anti-HPV18 E7/Protein E7 Polyclonal Antibody (VK556024) is a rabbit polyclonal antibody detecting Protein E7 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human papillomavirus 18 (HPV18)

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant HPV18 E7/Protein E7 (Met1-Gln105).

经测试应用

靶标

Protein E7, E7

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P06788

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Protein E7 is a ~11 kDa protein. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation.

1. Lau, L. et al. (2015) Science (New York, N.Y.) 350, 568-71. PMID: 26405230

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-E7 protein polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant HPV18 E7 (Catalog No: VK556062)

参考文献

产品说明书

Anti-HPV18 E7/Protein E7 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-HPV18 E7/Protein E7 Polyclonal Antibody (VK556024)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-HPV18 E7/Protein E7 Polyclonal Antibody (VK556024)

概述

图片

参考文献

产品说明书

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