Anti-cas13a Polyclonal Antibody (JN124014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

JN124014

品牌

abinScience

描述

Anti-cas13a Polyclonal Antibody (JN124014) is a rabbit polyclonal antibody detecting CRISPR-associated endoribonuclease Cas13a; EndoRNase; 3.1.-.-; LbaCas13a; cas13a in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Lachnospiraceae bacterium

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant cas13a (Val17-Asn319).

靶标

CRISPR-associated endoribonuclease Cas13a; EndoRNase; 3.1.-.-; LbaCas13a; cas13a

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P0DPB7

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

CRISPR-associated endoribonuclease Cas13a is a ~168 kDa protein. CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. Unlike many single-component effectors, this CRISPR-Cas system targets RNA. CRISPR clusters are transcribed from pre-CRISPR RNA (crRNA) and processed into crRNA by this protein. Cleaves linear target ssRNA in a pre-crRNA-dependent fashion, preferentially around A residues.

1. East-Seletsky, A. et al. (2017) Molecular cell 66, 373-383.e3. PMID: 28475872

Note

For research use only

图片

参考文献

产品说明书

Anti-cas13a Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-cas13a Polyclonal Antibody (JN124014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-cas13a Polyclonal Antibody (JN124014)

概述

图片

参考文献

产品说明书

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