Anti-pptT Polyclonal Antibody (JN126014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

JN126014

品牌

abinScience

描述

Anti-pptT Polyclonal Antibody (JN126014) is a rabbit polyclonal antibody detecting 4'-phosphopantetheinyl transferase PptT; PPTase; 2.7.8.7; pptT; Rv2794c in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Mycobacterium tuberculosis

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant pptT (Met1-Leu227).

靶标

4'-phosphopantetheinyl transferase PptT; PPTase; 2.7.8.7; pptT; Rv2794c

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

O33336

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

4'-phosphopantetheinyl transferase PptT is a ~24 kDa protein. Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein. Involved in post-translational modification of various type-I polyketide synthases required for the formation of both mycolic acids and lipid virulence factors. Acts on Pks13, Mas, PpsA, PpsB, PpsC and PpsD. Also acts on AcpM, the meromycolate extension acyl carrier protein. In addition, is involved in the activation of the acyl carrier protein MbtL and the nonribosomal peptides synthases MbtB and MbtE, which are involved in the biosynthesis of the siderophore mycobactin.

1. Chalut, C. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 8511-6. PMID: 16709676

2. Zimhony, O. et al. (2015) Biochemistry 54, 2360-71. PMID: 25785780

3. Jung, J. et al. (2016) FEBS open bio 6, 1220-1226. PMID: 28203522

4. Quadri, LE. et al. (1998) Chemistry & biology 5, 631-45. PMID: 9831524

Note

For research use only

图片

参考文献

产品说明书

Anti-pptT Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-pptT Polyclonal Antibody (JN126014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-pptT Polyclonal Antibody (JN126014)

概述

图片

参考文献

产品说明书

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