Anti-AIFM2/FSP1 Polyclonal Antibody (HC098014) is a rabbit polyclonal antibody detecting AIFM2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human
应用
ELISA, IHC, WB
宿主
Rabbit
克隆类型
Polyclonal
同种型
IgG
免疫原
E. coli - derived recombinant AIFM2/FSP1 (Met1-Pro373).
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Ferroptosis suppressor protein 1 (AIFM2/FSP1) is a ~40 kDa protein. A NAD(P)H-dependent oxidoreductase that acts as a key inhibitor of ferroptosis. At the plasma membrane, catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis. Acts in parallel to GPX4 to suppress phospholipid peroxidation and ferroptosis. This anti-ferroptotic function is independent of cellular glutathione levels. Also acts as a potent radical-trapping antioxidant by mediating warfarin-resistant vitamin K reduction in the canonical vitamin K cycle: catalyzes NAD(P)H-dependent reduction of vitamin K (phylloquinone, menaquinone-4 and menadione) to hydroquinone forms.
1. Doll, S. et al. (2019) Nature 575, 693-698. PMID: 31634899
2. Bersuker, K. et al. (2019) Nature 575, 688-692. PMID: 31634900
3. Mishima, E. et al. (2022) Nature 608, 778-783. PMID: 35922516
