Anti-HeV Nucleoprotein Polyclonal Antibody (VK432024)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

VK432024

品牌

abinScience

描述

Anti-Nucleoprotein Polyclonal Antibody (VK432024) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB. Suitable for Hendra virus and Nipah virus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Hendra virus (HeV), Nipah virus (NiV)

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant hendra virus Nucleoprotein (Met1-Ala394).

靶标

nucleocapsid, N

内毒素水平

Please contact with the lab for this information.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Purified by antigen affinity column.

Accession号

Q9IK92, O89339

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Nucleoprotein is a ~58 kDa protein. Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases. The nucleocapsid (NC) has a helical structure with either 13.4 N per turn, approximately 20 nm in diameter, with a hollow central cavity of 56 nm in diameter. The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis. Forms the encapsidation complex with the phosphoprotein protein P. Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P.

1. Ker, DS. et al. (2021) PLoS pathogens 17, e1009740. PMID: 34270629

2. Yabukarski, F. et al. (2014) Nature structural & molecular biology 21, 754-9. PMID: 25108352

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-Nucleocapsid-Hendra virus polyclonal antibody at 1ug/mL on various samples.
Lane 1: ATG18514 100ng

参考文献

产品说明书

Anti-HeV Nucleoprotein Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-HeV Nucleoprotein Polyclonal Antibody (VK432024)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-HeV Nucleoprotein Polyclonal Antibody (VK432024)

概述

图片

参考文献

产品说明书

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