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Anti-VP2 Polyclonal Antibody (VK079014)

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概述
货号VK079014
品牌abinScience
描述
Anti-VP2 Polyclonal Antibody (VK079014) is a rabbit polyclonal antibody detecting 1B VP2 mature peptide; 1B VP2 mature peptide in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
种属反应性Hepatitis A virus (HAV)
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant hav VP2 (Asp1-Gln222).
靶标1B VP2 mature peptide; 1B VP2 mature peptide
内毒素水平Please contact with the lab for this information.
纯化方式Purified by antigen affinity column.
Accession号P08617, Q67825, Q9DWR1, Q02381, P31788, Q05794, P13901, P06442, A5LGW7, P14553, Q5Y944, Q8V0N6, A3FMB2, P06441
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Genome polyprotein (VP2) is a ~251 kDa protein. Capsid proteins VP1, VP2, and VP3 form a closed capsid enclosing the viral positive strand RNA genome. All these proteins contain a beta-sheet structure called beta-barrel jelly roll. Together they form an icosahedral capsid (T=3) composed of 60 copies of each VP1, VP2, and VP3, with a diameter of approximately 300 Angstroms. VP1 is situated at the 12 fivefold axes, whereas VP2 and VP3 are located at the quasi-sixfold axes. The naked capsid interacts with the host receptor HAVCR1 to provide virion attachment to and probably entry into the target cell.

1. Wang, X. et al. (2015) Nature 517, 85-88. PMID: 25327248
2. Wang, X. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 770-775. PMID: 28074040
3. Silberstein, E. et al. (2001) Journal of virology 75, 717-25. PMID: 11134285
4. Costafreda, MI. et al. (2018) Journal of virology 92. PMID: 29437974
5. Feigelstock, D. et al. (1998) Journal of virology 72, 6621-8. PMID: 9658108
NoteFor research use only
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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