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Anti-MACV Nucleoprotein Polyclonal Antibody (VK765014)

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概述
货号VK765014
品牌abinScience
描述
Anti-MACV Nucleoprotein Polyclonal Antibody (VK765014) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB. Suitable for Machupo virus.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Machupo virus (MACV), Junin mammarenavirus (JUNV) (Junn mammarenavirus)
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant MACV Nucleoprotein (Met1-Leu564).
靶标Nucleoprotein, 3.1.13.-, Nucleocapsid protein, Protein N, N
内毒素水平Please contact with the lab for this information.
纯化方式Purified by antigen affinity column.
Accession号P26578, P14239
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Nucleoprotein is a ~63 kDa protein. Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of host IRF3, a protein involved in interferon activation pathway, leading to the inhibition of interferon-beta and IRF3-dependent promoters activation.

NoteFor research use only
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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