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Anti-ZEBOV VP35 Polyclonal Antibody (VK661014)

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概述
货号VK661014
品牌abinScience
描述
Anti-ZEBOV VP35 Polyclonal Antibody (VK661014) is a rabbit polyclonal antibody detecting VP35 in ELISA, IHC, WB. Suitable for Reston ebolavirus, Zaire ebolavirus, and Sudan ebolavirus.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Zaire ebolavirus (ZEBOV) (Zaire Ebola virus)
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant ZEBOV VP35 (Gly215-Ile340).
靶标Polymerase cofactor VP35, Ebola VP35, eVP35, VP35
内毒素水平Please contact with the lab for this information.
纯化方式Purified by antigen affinity column.
Accession号Q8JPY0, Q5XX07, Q05127, Q6V1Q9, Q91DE0
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Polymerase cofactor VP35 is a ~36 kDa protein. Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling. Acts as a polymerase cofactor in the RNA polymerase transcription and replication complexes. Serves as nucleoprotein/NP monomer chaperone prior to the formation of the large oligomeric RNA-bound complexes. Part of the external layer of the nucleocapsid, likely tethering the nucleocapsid to the matrix and membrane. Regulates RNA synthesis by modulating NP-RNA interactions and interacting with DYNLL1.

1. Kimberlin, CR. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 314-9. PMID: 20018665
NoteFor research use only
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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