Anti-SV40 Large T Antigen Polyclonal Antibody (VK099024)

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50ug
100ug

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概述
图片
参考文献
产品说明书

概述

货号

VK099024

品牌

abinScience

描述

Anti-SV40 Large T Antigen Polyclonal Antibody (VK099024) is a rabbit polyclonal antibody detecting Large T antigen in ELISA, IHC, WB. Suitable for Simian virus 40.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Simian virus 40 (SV40)

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant SV40 Large T Antigen (Gly251-Asp627).

靶标

Large T antigen, LT, LT-AG, 5.6.2.4, DNA 3'-5' helicase large T antigen

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P03070

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Large T antigen is a ~81 kDa protein. Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53.

1. Goetz, GS. et al. (1988) The Journal of biological chemistry 263, 383-92. PMID: 2826443

2. Wiekowski, M. et al. (1988) The Journal of biological chemistry 263, 436-42. PMID: 2826446

Note

For research use only

图片

Western blot

Lane 1: recombinant SV40 Large T Antigen (Catalog No: VK099022)

参考文献

产品说明书

Anti-SV40 Large T Antigen Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SV40 Large T Antigen Polyclonal Antibody (VK099024)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-SV40 Large T Antigen Polyclonal Antibody (VK099024)

概述

图片

参考文献

产品说明书

相关产品推荐

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