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Anti-RVFV Glycoprotein N Polyclonal Antibody (VK101014)

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概述
货号VK101014
品牌abinScience
描述
Anti-RVFV Glycoprotein N Polyclonal Antibody (VK101014) is a rabbit polyclonal antibody detecting glycoprotein in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
种属反应性Rift valley fever virus (RVFV)
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant RVFV Glycoprotein N (Glu154-Ser469).
靶标glycoprotein, glycoprotein
内毒素水平Please contact with the lab for this information.
纯化方式Purified by antigen affinity column.
Accession号P21401, P03518
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Envelopment polyprotein (Glycoprotein N) is a ~130 kDa protein. Structural component of the virion that interacts with glycoprotein C. It shields the hydrophobic fusion loops of the glycoprotein C, preventing premature fusion. The glycoprotein protrusions are arranged on an icosahedral lattice, with T=12 triangulation. They are able to attach the virion to the host cell receptor CD209/DC-SIGN and to promote fusion of membranes with the late endosome after endocytosis of the virion. Plays a role in the packaging of ribonucleoproteins and polymerase during virus assembly.

1. Freiberg, AN. et al. (2008) Journal of virology 82, 10341-8. PMID: 18715915
2. Halldorsson, S. et al. (2018) Nature communications 9, 349. PMID: 29367607
3. Lozach, PY. et al. (2011) Cell host & microbe 10, 75-88. PMID: 21767814
4. Piper, ME. et al. (2011) PloS one 6, e18070. PMID: 21445316
NoteFor research use only
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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