Anti-ASFV NP419L Polyclonal Antibody (VK110024)

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50ug
100ug

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概述
图片
参考文献
产品说明书

概述

货号

VK110024

品牌

abinScience

描述

Anti-ASFV NP419L Polyclonal Antibody (VK110024) is a rabbit polyclonal antibody detecting ASFV NP419L in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

African swine fever virus (ASFV)

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant ASFV NP419L (Met1-Ile419).

靶标

DNA ligase, EC:6.5.1.1, Polydeoxyribonucleotide synthase [ATP], LIG

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P35970, P0C990, P0C991, P26813, P0C992

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

DNA ligase (NP419L) is a ~48 kDa protein. Very low-fidelity DNA ligase that seals nicks in double-stranded DNA during DNA repair. Together with the viral repair DNA polymerase X, fills the single nucleotide gaps generated by the AP endonuclease. It is not essential for viral replication and recombination. Displays a very low adenylation activity towards DNA with 3'-dideoxy- or 3'-amino-terminated nicks compared to regular nick DNA.

1. Lamarche, BJ. et al. (2005) Biochemistry 44, 8408-17. PMID: 15938630

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-LIG polyclonal antibody at 1ug/mL on various samples.
Lane 1: ATG21816 100ng

参考文献

产品说明书

Anti-ASFV NP419L Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ASFV NP419L Polyclonal Antibody (VK110024)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ASFV NP419L Polyclonal Antibody (VK110024)

概述

图片

参考文献

产品说明书

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