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Anti-Human CD162/SELPLG Recombinant Antibody (SAA2392) (HC298013)

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规格:
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  • 100ug
  • 1mg
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概述
货号HC298013
品牌abinScience
种属反应性Human
应用ELISA, FCM
同种型IgG2a, kappa
克隆号SAA2392
靶标P-selectin glycoprotein ligand 1, PSGL-1, Selectin P ligand, CD162, SELPLG
内毒素水平<10 EU/mg
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号Q14242
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

P-selectin glycoprotein ligand 1 (CD162/SELPLG) is a ~43 kDa protein. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.

NoteFor research use only
Isotype ControlMouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
图片
  • SDS-PAGE

    SDS-PAGE for Anti-Human CD162/SELPLG Antibody (SAA2392)

  • Flow cytometry

    Flow-cytometry using APC anti-human CD162 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD162 monoclonal antibody (Catalog HC298013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

  • Flow cytometry

    Flow-cytometry using PE anti-human CD162 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD162 monoclonal antibody (Catalog HC298013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

  • Flow cytometry

    Flow-cytometry using FITC anti-human CD162 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD162 monoclonal antibody (Catalog HC298013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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