Anti-ACO1 Polyclonal Antibody (HB808014)

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50ug
100ug

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概述
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产品说明书

概述

货号

HB808014

品牌

abinScience

描述

Anti-ACO1 Polyclonal Antibody (HB808014) is a rabbit polyclonal antibody detecting ACO1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human ACO1 (Lys26-Ile243).

靶标

Cytoplasmic aconitate hydratase, Aconitase, EC:4.2.1.3, Citrate hydro-lyase, Ferritin repressor protein, Iron regulatory protein 1, IRP1, Iron-responsive element-binding protein 1, IRE-BP 1, ACO1, IREB1

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P21399, P28271, Q63270

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Cytoplasmic aconitate hydratase (ACO1) is a ~98 kDa protein. Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA.

1. Haile, DJ. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 11735-9. PMID: 1281544

2. Kaptain, S. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 10109-13. PMID: 1946430

3. Philpott, CC. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 7321-5. PMID: 8041788

4. Stehling, O. et al. (2013) Cell metabolism 18, 187-98. PMID: 23891004

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-ACO1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: U2OS cell lysate
Lane 3: 293T cell lysate
Lane 4: HepG2 cell lysate
Lane 5: A549 cell lysate
Lane 6: Mouse liver lysate
Lane 7: Rat liver lysate

参考文献

产品说明书

Anti-ACO1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ACO1 Polyclonal Antibody (HB808014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ACO1 Polyclonal Antibody (HB808014)

概述

图片

参考文献

产品说明书

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