Anti-CAPN1 Polyclonal Antibody (HC129014)

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50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HC129014

品牌

abinScience

描述

Anti-CAPN1 Polyclonal Antibody (HC129014) is a rabbit polyclonal antibody detecting CAPN1 in ELISA, IHC, WB. Suitable for Human, Rat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human CAPN1 (Gly27-Ser360).

靶标

Calpain 1, CANP 1, CANPL1, CAPN1, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 catalytic subunit, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, EC:3.4.22.52, Micromolar-calpain, muCANP

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P07384, P97571, O35350

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Calpain-1 catalytic subunit (CAPN1) is a ~81 kDa protein. Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'. Cleaves and activates caspase-7 (CASP7).

1. Gafni, J. et al. (2009) The Journal of biological chemistry 284, 25441-9. PMID: 19617626

2. Hsu, CY. et al. (2011) The Journal of biological chemistry 286, 23222-33. PMID: 21531719

3. Sorimachi, H. et al. (1990) Biological chemistry Hoppe-Seyler 371 Suppl, 171-6. PMID: 2400579

4. Ono, Y. et al. (2013) Journal of molecular biology 425, 2955-72. PMID: 23707407

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-CAPN1 polyclonal antibody at 1ug/mL on recombinant CAPN1 (Catalog No. HC129012).

参考文献

产品说明书

Anti-CAPN1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CAPN1 Polyclonal Antibody (HC129014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CAPN1 Polyclonal Antibody (HC129014)

概述

图片

参考文献

产品说明书

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