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Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody (VK457014)

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规格:
  • 50ug
  • 100ug
数量:
  • 概述

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  • 参考文献

  • 产品说明书

概述
货号VK457014
品牌abinScience
描述
Anti-HCoV-OC43 NP/Nucleoprotein Polyclonal Antibody (VK457014) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB. Suitable for Bovine coronavirus and Equine coronavirus.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Human coronavirus OC43 (HCoV-OC43)
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant HCoV-OC43 NP/Nucleoprotein (Met1-Ile448).
靶标N, NC, Nucleocapsid protein, Nucleoprotein, Protein N
内毒素水平Please contact with the lab for this information.
纯化方式Purified by antigen affinity column.
Accession号P33469
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Nucleoprotein (NP) is a ~49 kDa protein. Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions.

1. Dolliver, SM. et al. (2022) PLoS pathogens 18, e1011041. PMID: 36534661
NoteFor research use only
图片
  • Western blot

    Western blot analysis was performed using anti-NCAP-CVHOC polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant HCoV-OC43 NP/Nucleoprotein (Catalog No: VK457012)

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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