Anti-Human ATP5PB Polyclonal Antibody (HC151014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HC151014

品牌

abinScience

描述

Anti-Human ATP5PB Polyclonal Antibody (HC151014) is a rabbit polyclonal antibody detecting ATP5PB in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human ATP5PB (Ser96-Met256).

靶标

ATP synthase F(0) complex subunit B1, mitochondrial, ATP synthase peripheral stalk subunit b, mitochondrial, ATP synthase peripheral stalk-membrane subunit b, ATP synthase proton-transporting mitochondrial F(0) complex subunit B1, ATP synthase subunit b, ATP5F1, ATP5PB, ATPase subunit b

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P24539

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

别名

P24539

背景

ATP synthase peripheral stalk subunit b, mitochondrial (ATP5PB) is a ~28 kDa protein. Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro.

1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-ATP5PB polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse heart lysate
Lane 2: Mouse skeletal muscle lysate

参考文献

产品说明书

Anti-Human ATP5PB Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ATP5PB Polyclonal Antibody (HC151014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ATP5PB Polyclonal Antibody (HC151014)

概述

图片

参考文献

产品说明书

相关产品推荐

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